4D7U
The structure of the catalytic domain of NcLPMO9C from the filamentous fungus Neurospora crassa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-15 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.258, 66.755, 66.077 |
Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
Resolution | 64.805 - 1.560 |
R-factor | 0.158 |
Rwork | 0.156 |
R-free | 0.18760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eir |
RMSD bond length | 0.005 |
RMSD bond angle | 1.158 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.810 | 1.590 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.040 | |
Number of reflections | 49150 | |
<I/σ(I)> | 15.5 | 3.1 |
Completeness [%] | 91.3 | 29.2 |
Redundancy | 3.3 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.1 | 0.2M AMMONIUM CITRATE, 20% PEG 3350, PH 5.1 |