4D7T
Structure of the SthK Carboxy-Terminal Region in complex with cAMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2014-03-30 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 4 |
Unit cell lengths | 87.439, 87.439, 79.468 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.799 - 2.582 |
R-factor | 0.2677 |
Rwork | 0.265 |
R-free | 0.31000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u11 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.014 |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.800 | 2.720 |
High resolution limit [Å] | 2.580 | 2.580 |
Rmerge | 0.050 | |
Number of reflections | 18751 | |
Completeness [%] | 99.1 | 96.6 |
Redundancy | 3.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | PH 5 |