4D7S

Structure of the SthK Carboxy-Terminal Region in complex with cGMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	293
Detector technology	PIXEL
Collection date	2014-06-20
Detector	DECTRIS PILATUS 2M-F
Spacegroup name	I 4
Unit cell lengths	87.202, 87.202, 142.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.603 - 2.550
R-factor	0.2049
Rwork	0.203
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d7t
RMSD bond length	0.005
RMSD bond angle	1.117
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.610	2.680
High resolution limit [Å]	2.550	2.550
Rmerge	0.070
Number of reflections	17256
<I/σ(I)>	10.6
Completeness [%]	99.7	99.7
Redundancy	3.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		PH 7.5