4D7S
Structure of the SthK Carboxy-Terminal Region in complex with cGMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2014-06-20 |
Detector | DECTRIS PILATUS 2M-F |
Spacegroup name | I 4 |
Unit cell lengths | 87.202, 87.202, 142.337 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.603 - 2.550 |
R-factor | 0.2049 |
Rwork | 0.203 |
R-free | 0.24240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d7t |
RMSD bond length | 0.005 |
RMSD bond angle | 1.117 |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.610 | 2.680 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.070 | |
Number of reflections | 17256 | |
<I/σ(I)> | 10.6 | |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 3.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | PH 7.5 |