4D7R
Crystal structure of a chimeric protein with the Sec7 domain of Rickettsia prowazekii RalF and the capping domain of Legionella pneumophila RalF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-17 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.690, 94.840, 47.140 |
Unit cell angles | 90.00, 111.74, 90.00 |
Refinement procedure
Resolution | 24.010 - 1.800 |
R-factor | 0.1806 |
Rwork | 0.178 |
R-free | 0.22090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xsz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.790 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.080 | 0.670 |
Number of reflections | 33357 | |
<I/σ(I)> | 14.1 | 2.1 |
Completeness [%] | 95.7 | 92.6 |
Redundancy | 3.28 | 3.24 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2 M AMMONIUM CHLORIDE, 20% PEG 3350 |