4D6W

Crystal Structure of the low pH conformation of Chandipura Virus glycoprotein G ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-11
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 1 2 1
Unit cell lengths	365.590, 83.500, 60.820
Unit cell angles	90.00, 96.95, 90.00

Refinement procedure

Resolution	49.128 - 3.600
R-factor	0.1966
Rwork	0.193
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2CMZ
RMSD bond length	0.013
RMSD bond angle	1.571
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.130	3.730
High resolution limit [Å]	3.600	3.600
Rmerge	0.140	0.630
Number of reflections	21199
<I/σ(I)>	7.49	1.79
Completeness [%]	99.3	99.6
Redundancy	3.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	CRYSTALS WERE OBTAINED AT 293 K BY THE HANGING DROP VAPOUR DIFFUSION METHOD. DROPS WERE PREPARED BY MIXING 1 MICROLITER OF CHAV-GTH (4 MG/ML) SUPPLEMENTED WITH 0.2% N-DODECYL B-MALTOSIDE WITH 1 MICROLITER OF THE RESERVOIR SOLUTION (12% PEG 3350, 0.1 M SODIUM ACETATE PH 4.6) AND EQUILIBRATED AGAINST 500 MICROLITERS OF RESERVOIR SOLUTION.