Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.454, 87.420, 62.226 |
Unit cell angles | 90.00, 91.46, 90.00 |
Refinement procedure
Resolution | 40.441 - 1.482 |
R-factor | 0.1926 |
Rwork | 0.190 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4a6p |
RMSD bond length | 0.008 |
RMSD bond angle | 1.213 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.710 | 1.570 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.060 | 0.470 |
Number of reflections | 126096 | |
<I/σ(I)> | 8.76 | 2.02 |
Completeness [%] | 88.1 | 89.4 |
Redundancy | 1.92 | 1.92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.8 | 60 MM NA2HPO4 PH 6.0, 15% PEG 1000 |