4D6B

Human myelin protein P2, mutant P38G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Spacegroup name	P 41 21 2
Unit cell lengths	64.311, 64.311, 101.362
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.335 - 2.116
R-factor	0.2211
Rwork	0.218
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wut
RMSD bond length	0.004
RMSD bond angle	0.667
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.120	2.120
Rmerge	0.050	0.760
Number of reflections	12434
<I/σ(I)>	23.5	1.7
Completeness [%]	97.8	84.9
Redundancy	7.7	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1