4D64
Structure of porin Omp-Pst1 from P. stuartii; the crystallographic symmetry generates a dimer of trimers.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-27 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.520, 142.020, 113.320 |
Unit cell angles | 90.00, 131.00, 90.00 |
Refinement procedure
Resolution | 36.254 - 3.200 |
R-factor | 0.2102 |
Rwork | 0.207 |
R-free | 0.26110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MODELLER-MODEL OF OMP-PST1 BASED ON PDB ENTRY 1OPF |
RMSD bond length | 0.006 |
RMSD bond angle | 1.007 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.280 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.120 | 0.590 |
Number of reflections | 27279 | |
<I/σ(I)> | 10.98 | 2 |
Completeness [%] | 90.5 | 93.2 |
Redundancy | 2.68 | 2.66 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 14% PEG6000 MME, 0.1 M BUFFER MES PH6.5, 0.1 M MGCL2 |