4D5L
Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK II |
EM Data Collection
Microscope | FEI TECNAI F20 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2 |
Detector | KODAK SO-163 FILM |
Number of Images | 366 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 9 |
Software | UCSF Chimera,SPARX,SPIDER |
Symmetry | C1 |