4D5H
Focal Adhesion Kinase catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-13 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.892, 123.587, 50.803 |
| Unit cell angles | 90.00, 95.00, 90.00 |
Refinement procedure
| Resolution | 50.660 - 1.750 |
| R-factor | 0.18264 |
| Rwork | 0.181 |
| R-free | 0.20503 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jkk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.312 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.790 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.030 | 0.370 |
| Number of reflections | 55431 | |
| <I/σ(I)> | 11.8 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM TRIS PH 8.5, 75 MM LISO4, 22% PEG 4000, 10 MM TCEP |
