4D5F
tetracycline repressor class H, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2014-10-08 |
| Detector | RIGAKU CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.456, 109.037, 69.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.520 - 2.200 |
| R-factor | 0.19116 |
| Rwork | 0.189 |
| R-free | 0.25146 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d5c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.287 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.110 | 0.490 |
| Number of reflections | 11494 | |
| <I/σ(I)> | 12.1 | 2.7 |
| Completeness [%] | 99.0 | 96.2 |
| Redundancy | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 1.5 M LI2SO4, 0.1 M TRIS PH 8.5, CRYO: BRIEF SOAK IN 1.5 M LI2SO4, 0.1 M TRIS PH 8.5, 10% GLYCEROL |
