4D5D
Crystal structure of CymA from Klebsiella oxytoca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 140.160, 77.991, 109.927 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.100 - 1.902 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.19760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v3g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.157 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 1.100 |
| Number of reflections | 94765 | |
| <I/σ(I)> | 12.9 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.4 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | MORPHEUS 1/30, 0.09 M NPS, 0.1 M BUFFER SYSTEM 2 PH 7.5, 20% ETHYLENE GLYCOL, 10% PEG 8000, |
