4D55
Focal Adhesion Kinase catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.011, 44.495, 66.989 |
| Unit cell angles | 90.00, 95.00, 90.00 |
Refinement procedure
| Resolution | 44.880 - 2.300 |
| R-factor | 0.20137 |
| Rwork | 0.198 |
| R-free | 0.25712 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jkk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.334 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.730 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.260 |
| Number of reflections | 11957 | |
| <I/σ(I)> | 11.2 | 2.9 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM TRIS PH 8.5, 300 MM LISO4, 24% PEG4000, 10 MM TCEP |
