4D4V
Focal Adhesion Kinase catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.996, 123.597, 51.008 |
| Unit cell angles | 90.00, 94.49, 90.00 |
Refinement procedure
| Resolution | 50.850 - 2.100 |
| R-factor | 0.19365 |
| Rwork | 0.192 |
| R-free | 0.22704 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jkk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.231 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.850 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.030 | 0.180 |
| Number of reflections | 32413 | |
| <I/σ(I)> | 15.6 | 4.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM TRIS PH 8.5, 75 MM LISO4, 22% PEG4000, 10 MM TCEP |
