4D4T
RSV Matrix protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-27 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 87.127, 87.127, 144.721 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.453 - 1.900 |
R-factor | 0.1836 |
Rwork | 0.182 |
R-free | 0.20560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vqp |
RMSD bond length | 0.016 |
RMSD bond angle | 1.038 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.450 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.100 | 0.750 |
Number of reflections | 25922 | |
<I/σ(I)> | 13.5 | 3.2 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 5.5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 30% GLYCEROL 0.1 M MES (6.5) 1.8 M AM2SO4 |