4D4T
RSV Matrix protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 87.127, 87.127, 144.721 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.453 - 1.900 |
| R-factor | 0.1836 |
| Rwork | 0.182 |
| R-free | 0.20560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vqp |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.038 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.450 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.750 |
| Number of reflections | 25922 | |
| <I/σ(I)> | 13.5 | 3.2 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 30% GLYCEROL 0.1 M MES (6.5) 1.8 M AM2SO4 |
