4D4E
Crystal structure of computationally designed armadillo repeat proteins for modular peptide recognition.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-17 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.810, 68.670, 126.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.350 - 2.000 |
| R-factor | 0.19119 |
| Rwork | 0.188 |
| R-free | 0.26342 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ARM00009 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.875 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.120 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.590 |
| Number of reflections | 27662 | |
| <I/σ(I)> | 9.3 | 2.3 |
| Completeness [%] | 88.2 | 72.3 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | PH 5.5 0.3 M NA-ACETATE/ 0.1 M NA- ACETATE/ 25 % PEG 2K MME |
