4D47

X-ray structure of the levansucrase from Erwinia amylovora

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-18
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 2
Unit cell lengths	155.589, 178.736, 158.806
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.851 - 2.770
R-factor	0.2638
Rwork	0.262
R-free	0.29780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w18
RMSD bond length	0.004
RMSD bond angle	0.958
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	BALBES
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	178.740	2.920
High resolution limit [Å]	2.770	2.770
Rmerge	0.180	0.590
Number of reflections	112787
<I/σ(I)>	3	3
Completeness [%]	99.9	99.9
Redundancy	6	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M POTASSIUM THIOCYANATE, 30% W/V PEG 2000 MME