4D3Y
The structure of inactive prolegumain from chinese hamster.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.270, 60.330, 153.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.941 - 2.400 |
| R-factor | 0.1714 |
| Rwork | 0.168 |
| R-free | 0.24130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fgu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.139 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.230 | 1.220 |
| Number of reflections | 14574 | |
| <I/σ(I)> | 10.7 | 2.33 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 12.9 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.03 M MGCL2, 0.03 M CACL2, 10%(W/V) PEG 20000, 20%(V/V) PEG 550 MME, 0.1 MOPS/HEPES PH 7.5 |
