4D3R

Crystal structure of point mutated DUSP19 (I187A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-06
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	47.396, 47.934, 57.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.670
R-factor	0.16272
Rwork	0.161
R-free	0.19905
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s4e
RMSD bond length	0.022
RMSD bond angle	2.025
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.670
High resolution limit [Å]	1.640	1.640
Rmerge	0.060	0.080
Number of reflections	16565
<I/σ(I)>		32.2
Completeness [%]	95.9	96.6
Redundancy	13.3	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				1.9M AMMONIUM SULFATE