4D3P
crystal structure of point mutated DUSP19 (C150A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-06 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.183, 50.147, 57.687 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.270 |
R-factor | 0.15624 |
Rwork | 0.155 |
R-free | 0.17762 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s4e |
RMSD bond length | 0.024 |
RMSD bond angle | 2.277 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.200 |
High resolution limit [Å] | 1.180 | 1.180 |
Rmerge | 0.080 | 0.360 |
Number of reflections | 45782 | |
<I/σ(I)> | 6.8 | 5.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1.8M AMMONIUM SULFATE |