4D3E
Tetramer of IpaD, modified from 2J0O, fitted into negative stain electron microscopy reconstruction of the wild type tip complex from the type III secretion system of Shigella flexneri
EM specimen
| Staining | Yes |
| Staining material | uranyl acetate |
EM Data Collection
| Microscope | FEI TECNAI 20 |
| Electron Gun | LAB6 |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | FEI EAGLE (4k x 4k) |
| Number of Images | 300 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 24 |
| Software | UCSF Chimera,EMAN,SPIDER |
| Symmetry | C1 |
