4D2X
Negative-stain electron microscopy of E. coli ClpB of Y503D hyperactive mutant (BAP form bound to ClpP)
EM specimen
| Staining | Yes |
| Staining material | uranyl acetate |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 108 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 20 |
| Software | UCSF Chimera,IMAGIC,SPIDER |
| Symmetry | C6 |
