4D2K

Crystal structure of DREP2 CIDE domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-16
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	50.283, 88.708, 113.372
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.263 - 2.302
R-factor	0.2199
Rwork	0.216
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2eel
RMSD bond length	0.009
RMSD bond angle	1.381
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.010	0.280
Number of reflections	22067
<I/σ(I)>	16.2	5.2
Completeness [%]	95.4	98.3
Redundancy	9.7	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.2		100 MM BIS-TRIS PH6.2, 300 MM MAGNESIUM FORMATE DIHYDRATE