4D2G
Crystal structure of human PCNA in complex with p15 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.053, 41.228, 141.121 |
Unit cell angles | 90.00, 105.39, 90.00 |
Refinement procedure
Resolution | 44.350 - 2.650 |
R-factor | 0.1744 |
Rwork | 0.170 |
R-free | 0.24990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vym |
RMSD bond length | 0.016 |
RMSD bond angle | 1.786 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.350 | 2.740 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.090 | 0.510 |
Number of reflections | 24870 | |
<I/σ(I)> | 9.3 | 2 |
Completeness [%] | 98.8 | 89.1 |
Redundancy | 3.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 40% PEG 300 IN 0.1 MM PHOSPHATE-CITRATE PH 4.2 |