4D2C
Structure of a di peptide bound POT family peptide transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-18 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 102.060, 110.330, 110.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.030 - 2.470 |
R-factor | 0.2246 |
Rwork | 0.222 |
R-free | 0.26580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4aps |
RMSD bond length | 0.003 |
RMSD bond angle | 0.757 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.030 | 2.530 |
High resolution limit [Å] | 2.470 | 2.470 |
Rmerge | 0.070 | 0.640 |
Number of reflections | 22160 | |
<I/σ(I)> | 10.9 | 2 |
Completeness [%] | 97.9 | 99.4 |
Redundancy | 4.1 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 16-23 %(V/V) PEG 400, 0.1 M HEPES PH 7.0, 0.15-0.55 M NH4H2PO4 AND 10 MM ALA-PHE. CRYSTALS WERE GROWN BY THE LCP METHOD USING 7.8 MAG AS HOSTING LIPID. |