4D2B
Structure of a ligand free POT family peptide transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 102.190, 110.190, 110.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.099 - 2.350 |
| R-factor | 0.2145 |
| Rwork | 0.213 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aps |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.827 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.100 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.170 | 0.710 |
| Number of reflections | 26241 | |
| <I/σ(I)> | 8.3 | 2.2 |
| Completeness [%] | 99.3 | 98 |
| Redundancy | 6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | 16-23 %(V/V) PEG 400, 0.1 M HEPES PH 7.0 AND 0.15-0.55 M NH4H2PO4. CRYSTALS WERE GROWN BY THE LCP METHOD USING 7.8 MAG AS HOSTING LIPID. |
