4D1V

A F218Y mutant of VIM-7 from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 43
Unit cell lengths	69.947, 69.947, 46.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.730 - 1.700
R-factor	0.1613
Rwork	0.159
R-free	0.19650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y8b
RMSD bond length	0.009
RMSD bond angle	1.158
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.060	0.560
Number of reflections	25160
<I/σ(I)>	16.3	2.6
Completeness [%]	100.0	100
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				25% PEG 3350, 0.1 M BISTRIS PH 5.5, 0.2 M NACL