4D1Q
Hermes transposase bound to its terminal inverted repeat
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 95 |
Collection date | 2011-02-17 |
Detector | RIGAKU IMAGING PLATE |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 265.150, 265.150, 157.640 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 3.400 |
R-factor | 0.2118 |
Rwork | 0.212 |
R-free | 0.25430 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.016 |
RMSD bond angle | 2.000 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 3.300 |
Rmerge | 0.180 |
Number of reflections | 94693 |
<I/σ(I)> | 12.54 |
Completeness [%] | 98.7 |
Redundancy | 13.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 3 UL OF THE HERMES 79-612/L16-1T COMPLEX AT 8 MG/ML PROTEIN WAS MIXED WITH 1 UL WELL SOLUTION CONSISTING OF 0.18 M KCL, 5 MM DTT, 0.8 M K, NA TARTRATE, 0.1 M MES PH 6.5, AND 5% (V/V) T-BUTANOL. |