4D1M

Tetramerization domain of zebrafish p53 (crystal form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	70.861, 74.507, 74.945
Unit cell angles	90.00, 117.79, 90.00

Refinement procedure

Resolution	28.900 - 2.200
R-factor	0.22845
Rwork	0.226
R-free	0.27996
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.336
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.900	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.040	0.520
Number of reflections	34925
<I/σ(I)>	14.8	2.5
Completeness [%]	99.3	99.5
Redundancy	3.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	SITTING DROP VAPOR DIFFUSION AT 20 DEGREE C; PROTEIN SOLUTION: 12 MG/ML IN 20 MM TRIS PH 7.5, 50 MM NACL, 5 MM DTT; CRYSTALLIZATION BUFFER: 160 MM ZINC ACETATE, 80 MM SODIUM CACODYLATE, PH 6.5, 12% (W/V) POLYETHYLENE GLYCOL 8,000 AND 19% (W/V) GLYCEROL