4D1L
Tetramerization domain of zebrafish p53 (crystal form I)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 87.853, 120.611, 61.029 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.500 - 1.970 |
R-factor | 0.21876 |
Rwork | 0.217 |
R-free | 0.24698 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c26 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.342 |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.500 | 2.080 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.050 | 0.650 |
Number of reflections | 23226 | |
<I/σ(I)> | 18.1 | 2.8 |
Completeness [%] | 99.6 | 99.3 |
Redundancy | 5.5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | SITTING DROP VAPOR DIFFUSION AT 20 DEGREE C; PROTEIN SOLUTION: 12 MG/ML IN 20 MM TRIS PH 7.5, 50 MM NACL, 5 MM DTT; CRYSTALLIZATION BUFFER: 50 MM ZINC ACETATE AND 20% (W/V) POLYETHYLENE GLYCOL 3,350. |