4D1I
The structure of the GH35 beta-galactosidase Bgl35A from Cellvibrio japonicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-26 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 98.911, 115.783, 116.036 |
Unit cell angles | 90.21, 90.25, 90.38 |
Refinement procedure
Resolution | 116.030 - 1.800 |
R-factor | 0.18617 |
Rwork | 0.185 |
R-free | 0.21153 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u7v |
RMSD bond length | 0.011 |
RMSD bond angle | 1.476 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 0.630 |
Number of reflections | 459893 | |
<I/σ(I)> | 8.7 | 1.1 |
Completeness [%] | 96.4 | 95.1 |
Redundancy | 1.8 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 2.8 M SODIUM ACETATE PH 7.0 |