4D1F
Crystal structure of the fiber head domain of the Atadenovirus snake adenovirus 1, native, first P212121 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-30 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.660, 122.450, 133.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.890 - 2.700 |
| R-factor | 0.21442 |
| Rwork | 0.212 |
| R-free | 0.24736 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d0v |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.251 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.170 | 0.880 |
| Number of reflections | 36484 | |
| <I/σ(I)> | 10.1 | 2.8 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 9.3 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10 MM TRIS-HCL, 1.7 M AMMONIUM SULFATE, 0.085 M HEPES SODIUM SALT PH 7.5, 1.7%(V/V) PEG 400, 15%(V/V) GLYCEROL |
