4D1E
THE CRYSTAL STRUCTURE OF HUMAN MUSCLE ALPHA-ACTININ-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-24 |
Detector | MARRESEARCH |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 73.334, 102.613, 182.977 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.307 - 3.500 |
R-factor | 0.2074 |
Rwork | 0.205 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1WKU 1hci 1h8b |
RMSD bond length | 0.011 |
RMSD bond angle | 1.592 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.310 | 3.830 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.200 | 1.300 |
Number of reflections | 18047 | |
<I/σ(I)> | 11.2 | |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 8.5 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2 M MG FORMATE 5% PEG SMEAR, 0.01 M EDTA |