4D1E

THE CRYSTAL STRUCTURE OF HUMAN MUSCLE ALPHA-ACTININ-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-24
Detector	MARRESEARCH
Spacegroup name	P 2 21 21
Unit cell lengths	73.334, 102.613, 182.977
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.307 - 3.500
R-factor	0.2074
Rwork	0.205
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1WKU 1hci 1h8b
RMSD bond length	0.011
RMSD bond angle	1.592
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.310	3.830
High resolution limit [Å]	3.500	3.500
Rmerge	0.200	1.300
Number of reflections	18047
<I/σ(I)>	11.2
Completeness [%]	99.8	99.8
Redundancy	8.5	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.2 M MG FORMATE 5% PEG SMEAR, 0.01 M EDTA