4D1D
STRUCTURE OF MHP1, A NUCLEOBASE-CATION-SYMPORT-1 FAMILY TRANSPORTER with the inhibitor 5-(2-naphthylmethyl)-L-hydantoin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2011-09-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.600, 106.900, 106.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.777 - 3.700 |
| R-factor | 0.2606 |
| Rwork | 0.256 |
| R-free | 0.30770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d1a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.274 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 3.800 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.060 | 0.690 |
| Number of reflections | 10714 | |
| <I/σ(I)> | 8.3 | 2 |
| Completeness [%] | 97.0 | 96 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PROTEIN WAS CRYSTALLIZED FROM 27-33 % PEG 300, 0.1 M NACL AND 0.1M NA-PHOSPHATE (PH 7.0). NMH WAS ADDED TO THE DROP AS A SATURATED SOLUTION. |
