4D1A
STRUCTURE OF MHP1, A NUCLEOBASE-CATION-SYMPORT-1 FAMILY TRANSPORTER, IN A CLOSED CONFORMATION WITH INDOLYLMETHYL-HYDANTOIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 287 |
Detector technology | CCD |
Collection date | 2011-02-23 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.430, 106.360, 100.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.534 - 3.400 |
R-factor | 0.2494 |
Rwork | 0.247 |
R-free | 0.28260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jln |
RMSD bond length | 0.007 |
RMSD bond angle | 1.202 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.500 | 3.490 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.050 | 0.880 |
Number of reflections | 12215 | |
<I/σ(I)> | 13.2 | 1.2 |
Completeness [%] | 92.0 | 87 |
Redundancy | 4.1 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | PROTEIN WAS CRYSTALLIZED IN 27-33 % PEG 300, 0.1M NACL AND 0.1M NA-PHOSPHATE (PH 7.0). INDOLYLMETHYL-HYDANTOIN WAS ADDED TO THE DROP AS A SATURATED SOLUTION. |