4D0V

Crystal structure of the fiber head domain of the Atadenovirus snake adenovirus 1, native, I213 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-10
Detector	ADSC QUANTUM 210
Spacegroup name	I 21 3
Unit cell lengths	149.620, 149.620, 149.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.168
Rwork	0.167
R-free	0.20125
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.013
RMSD bond angle	1.513
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.060	0.400
Number of reflections	60984
<I/σ(I)>	17.3	7.2
Completeness [%]	99.9	100
Redundancy	6.2	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		10 MM TRIS-HCL, 1.7 M AMMONIUM SULFATE, 0.085 M HEPES SODIUM SALT PH 7.5, 1.7%(V/V) POLYETHYLENE GLYCOL (PEG) 400, 15%(V/V) GLYCEROL