4D0G
Structure of Rab14 in complex with Rab-Coupling Protein (RCP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 95.870, 148.228, 38.808 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.500 - 2.500 |
R-factor | 0.23113 |
Rwork | 0.224 |
R-free | 0.29438 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4drz |
RMSD bond length | 0.008 |
RMSD bond angle | 1.302 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.100 | 0.800 |
Number of reflections | 9973 | |
<I/σ(I)> | 8.5 | 1.72 |
Completeness [%] | 96.5 | 70.1 |
Redundancy | 8.9 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.75 | 14% PEG 8000, 0.1 M HEPES PH 6.75 |