4CZZ
Histone demethylase LSD1(KDM1A)-CoREST3 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 118.040, 177.440, 235.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 141.780 - 3.000 |
| R-factor | 0.1973 |
| Rwork | 0.197 |
| R-free | 0.23612 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v1d |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.731 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.890 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.190 | 0.820 |
| Number of reflections | 49662 | |
| <I/σ(I)> | 6.5 | 1.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
