4CZW

Structure of the Neurospora crassa Pan2 catalytic unit (protease and nuclease domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99999, 0.97978, 1.28082, 1.28639
Spacegroup name	P 61 2 2
Unit cell lengths	85.565, 85.565, 471.793
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.140 - 2.600
R-factor	0.2108
Rwork	0.209
R-free	0.25350
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.002
RMSD bond angle	0.540
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELX
Refinement software	PHENIX ((PHENIX.REFINE 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.180	2.720
High resolution limit [Å]	2.600	2.600
Number of reflections	32906
<I/σ(I)>	13.4	1.9
Completeness [%]	99.9	99.9
Redundancy	6.4	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		1.4M SODIUM FORMATE, 100MM SODIUM ACETATE, 100MM SODIUM CITRATE (PH5.5)