4CZQ
Crystal structure of the extralong fungal manganese peroxidase from Ceriporiopsis subvermispora in complex with cadmium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 108.620, 108.620, 68.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.349 - 1.198 |
| R-factor | 0.1514 |
| Rwork | 0.151 |
| R-free | 0.16190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3MQ5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.379 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.270 | 1.270 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.050 | 1.290 |
| Number of reflections | 128714 | |
| <I/σ(I)> | 0.19 | 1.1 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 8.2 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 18% PEG 4000, 100MM NA-ACETATE PH 4.6 |
