4CZ6

Truncated tetramerization domain of zebrafish p53 (crystal form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Spacegroup name	P 31 2 1
Unit cell lengths	57.529, 57.529, 96.875
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.820 - 1.530
R-factor	0.15003
Rwork	0.148
R-free	0.18365
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.100
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.800	1.610
High resolution limit [Å]	1.530	1.530
Rmerge	0.040	0.530
Number of reflections	28720
<I/σ(I)>	25.8	3.5
Completeness [%]	100.0	100
Redundancy	8	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	SITTING DROP VAPOR DIFFUSION AT 20 DEGREE C; PROTEIN SOLUTION: 17 MG/ML IN 20 MM TRIS PH 7.5, 50 MM NACL, 5 MM DTT; CRYSTALLIZATION BUFFER: 50% (W/V) POLYETHYLENE GLYCOL 200, 100 MM TRIS, PH 7.0