4CZ5

Truncated tetramerization domain of zebrafish p53 (crystal form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	33.232, 33.685, 101.805
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.100 - 1.020
R-factor	0.15866
Rwork	0.158
R-free	0.17846
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.339
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.090	1.080
High resolution limit [Å]	1.020	1.020
Rmerge	0.030	0.540
Number of reflections	59076
<I/σ(I)>	19.3	3
Completeness [%]	99.8	99.9
Redundancy	5.2	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	SITTING DROP VAPOR DIFFUSION AT 20 DEGREE C; PROTEIN SOLUTION: 17 MG/ML IN 20 MM TRIS PH 7.5, 50 MM NACL, 5 MM DTT; CRYSTALLIZATION BUFFER: 2.4 M AMMONIUM SULFATE, 100 MM BICINE, PH 9.0