4CYZ
Structure of the A_mallard_Sweden_51_2002 H10 Avian Haemmaglutinin in complex with avian receptor analog LSTA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.070, 230.580, 68.460 |
| Unit cell angles | 90.00, 110.41, 90.00 |
Refinement procedure
| Resolution | 115.290 - 2.400 |
| R-factor | 0.20724 |
| Rwork | 0.205 |
| R-free | 0.24389 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cyv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.094 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.860 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.110 | 0.700 |
| Number of reflections | 75050 | |
| <I/σ(I)> | 9.5 | 4 |
| Completeness [%] | 99.1 | 99.2 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18 PERCENT PEG 2000MME AND 0.1 M BICINE PH 9.0 |
