4CYC
CRYSTAL STRUCTURE OF A UBX-EXD-DNA COMPLEX INCLUDING THE HEXAPEPTIDE AND UBDA MOTIFS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 97.707, 97.707, 73.735 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.360 |
| R-factor | 0.2128 |
| Rwork | 0.212 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4BZL |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | TRUNCATE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.620 | 2.370 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.040 | 0.970 |
| Number of reflections | 17094 | |
| <I/σ(I)> | 41.2 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.8 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M AMMONIUM PHOSPHATE, pH 7.5 |
