4CXV
Structure of bifunctional endonuclease (AtBFN2) in complex with phosphate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-26 |
Detector | MARRESEARCH MX-300 |
Spacegroup name | P 1 |
Unit cell lengths | 45.524, 52.870, 60.841 |
Unit cell angles | 71.34, 78.63, 76.62 |
Refinement procedure
Resolution | 25.430 - 2.000 |
R-factor | 0.20423 |
Rwork | 0.202 |
R-free | 0.24942 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cwm |
RMSD bond length | 0.010 |
RMSD bond angle | 1.563 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.260 |
Number of reflections | 34563 | |
<I/σ(I)> | 9.4 | 3.3 |
Completeness [%] | 96.7 | 95.2 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M HEPES, PH 7.5 0.2 M NA2HPO4 30% (W/V) PEG3350 |