4CXP
Structure of bifunctional endonuclease (AtBFN2) from Arabidopsis thaliana in complex with sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-09 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.140, 66.819, 99.556 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.340 - 1.220 |
R-factor | 0.11947 |
Rwork | 0.119 |
R-free | 0.13444 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cwm |
RMSD bond length | 0.009 |
RMSD bond angle | 1.621 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.260 |
High resolution limit [Å] | 1.220 | 1.220 |
Rmerge | 0.060 | 0.460 |
Number of reflections | 107958 | |
<I/σ(I)> | 35.3 | 3.5 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 11.7 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M HEPES PH 7.5, 0.2 M LI2SO4, 30% (W/V) PEG3350 |