4CXP
Structure of bifunctional endonuclease (AtBFN2) from Arabidopsis thaliana in complex with sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-09 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.140, 66.819, 99.556 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.340 - 1.220 |
| R-factor | 0.11947 |
| Rwork | 0.119 |
| R-free | 0.13444 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cwm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.621 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.260 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 107958 | |
| <I/σ(I)> | 35.3 | 3.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 11.7 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 0.2 M LI2SO4, 30% (W/V) PEG3350 |
