4CXF
Structure of CnrH in complex with the cytosolic domain of CnrY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 61.010, 79.122, 80.537 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.561 - 1.750 |
| R-factor | 0.1754 |
| Rwork | 0.174 |
| R-free | 0.20090 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.071 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.560 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.040 | 0.490 |
| Number of reflections | 19854 | |
| <I/σ(I)> | 20.4 | 2.5 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2.0 TO 2.2 M AMMONIUM SULFATE, 100 MM HEPES PH 7.5 AND 2 % PEG 400 |
