4CX9
The 5-coordinate proximal NO complex of cytochrome c prime from Shewanella frigidimarina
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-15 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.300, 58.279, 76.137 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.280 - 1.430 |
R-factor | 0.16232 |
Rwork | 0.161 |
R-free | 0.18631 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yli |
RMSD bond length | 0.016 |
RMSD bond angle | 2.131 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.100 | 1.510 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.080 | 0.770 |
Number of reflections | 46165 | |
<I/σ(I)> | 12.6 | 2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 2.2M AM SULFATE, 0.1M HEPES PH7 |