4CX8
Monomeric pseudorabies virus protease pUL26N at 2.5 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-02 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.437, 101.337, 130.078 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.940 - 2.530 |
R-factor | 0.20078 |
Rwork | 0.198 |
R-free | 0.25215 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DIMERIC PRV PROTEASE YET TO BE DEPOSITED |
RMSD bond length | 0.014 |
RMSD bond angle | 1.788 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.680 |
High resolution limit [Å] | 2.530 | 2.530 |
Rmerge | 0.080 | 0.660 |
Number of reflections | 18230 | |
<I/σ(I)> | 14.29 | 2.06 |
Completeness [%] | 97.8 | 93.6 |
Redundancy | 3.8 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.1 M TRIS PH 8, 14% PEG 20,000 |