4CVU
Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 166.456, 166.456, 121.452 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 117.700 - 1.900 |
R-factor | 0.23498 |
Rwork | 0.234 |
R-free | 0.26254 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.013 |
RMSD bond angle | 1.511 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | HKL2Map |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.300 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.130 | 1.340 |
Number of reflections | 131814 | |
<I/σ(I)> | 7.9 | 1.7 |
Completeness [%] | 99.1 | 96.9 |
Redundancy | 7.4 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 26% PEG 400, 0.13 M CDCL2, 0.1 M SODIUM ACETATE PH 4.7 |