4CVU
Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 166.456, 166.456, 121.452 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 117.700 - 1.900 |
| R-factor | 0.23498 |
| Rwork | 0.234 |
| R-free | 0.26254 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.511 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.130 | 1.340 |
| Number of reflections | 131814 | |
| <I/σ(I)> | 7.9 | 1.7 |
| Completeness [%] | 99.1 | 96.9 |
| Redundancy | 7.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 26% PEG 400, 0.13 M CDCL2, 0.1 M SODIUM ACETATE PH 4.7 |
